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Human Hematopoetic Malignancies Cell Lines

GryDiv

WSU-WM
Established from pleural effusion from a patient with IgMκ Waldenstrom's macroglobulinemia (WM) which is a human B-cell tumor.

WSU-FSCCL
A human B-cell line, was established from an EBV-Negative low-grade follicular small cleaved cell lymphoma in leukemic phase.

WSU-DLCL2
A human diffuse large cell lymphoma cell line, is a mature B-cell line (IgG lambda) that is negative for EBV nuclear antigen, expresses the multidrug resistance phenotype, and has t(14;18)(q32;q21) plus other chromosomal aberrations.

WSU-Pre-B-ALL
A new human EBV negative pre-B-acute lymphoblastic Leukemia. It was established from a frozen peripheral blood of a patient with ALL in leukemic phase. Both the frozen cells and the established cell line exhibit t (1; 19) chromosomal translocation, partial deletion of the short arm (p) of chromosome 9 and a loss of chromosome 13 as clonal abnormalities.

WSU-AML
A new human aggressive AML cell line. It was established from a frozen peripheral blood of a patient with AML. The cell line showed a partial interstitial deletion of the long arm (q) of chromosome 9. Phenotypically, the cell line is consistent with a diagnosis of M7 AML.


Cell line Image (10x) Doubling time Seeding density (cell/mL)
WSU-AML WSU-AML 35h 2 x 105 cells /mL
WSU-NHL WSU-NHL 42h 2 x 105 cells /mL
WSU-DLCL2 WSU-DLCL2 22h 0.25 – 0.5 x 105 cells /mL
WSU-WM WSU-WM 18h 0.25 – 0.5 x 105 cells /mL
WSU-FSCCL WSU-FSCCL 27h 1 - 2 x 105 cells /mL

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