|
|
|
Autoradiography Services
|
 |
XpressWayTM Profiles and CustomMappingTM provide a high level assessment of the expression patterns of genes in human tissue. However, it is also important to identify the pattern of expression of a target at the cellular level in order to gain a greater understanding of the potential function of that target and its possible role in disease.
In addition to looking at localization of the target mRNA (ISH) or protein (IHC), Asterand can perform ligand binding autoradiography to visualize the interaction between a high affinity radiolabelled ligand and its binding sites in sections of frozen human tissue. This approach enables localization of binding sites to specific cell types or tissue structures.
Asterand's specific expertise in ligand autoradiography supports drug discovery in CNS.
 |
| Binding of the tritiated cannabinoid receptor agonist, [3H]-CP55,940, to sections of human hippocampus (panel A) and basal ganglia (panel C). Dark grey areas represent high levels of binding. Non-specific binding of [3H]-CP55,940 is defined in adjacent sections of hippocampus (panel B) and basal ganglia (panel D) incubated in the presence of excess unlabelled CP55,940. |
Localization of ligand binding sites using autoradiography
- For known receptor radioligands, donor tissues (frozen sections) incubated with radioligand at the published Kd
- For novel receptor radioligands, Kd and Bmax determined in donor tissues (frozen sections) through generation of a concentration-effect curve in the presence and absence of excess non-labelled ligand
- Binding can be quantified by comparison to grey-scale standard curves
- Adjacent sections are stained using a histochemical stain, for example acetylcholinesterase, cresyl violet or methylene blue
For further information, contact us at advantage@asterand.com
|
E-Newsletter
